In bacteria adenine in the DNA sequence GATC can be methylated at N6.
a) Draw the structures of deoxyadenosine and 6-methyl deoxyadenosine
b) Draw the hydrogen bonding pattern between deoxyadenosine and deoxythymidine and 6-methyl deoxyadenosine and deoxythymidine.
c) Does the methylation impact the base pairing stability? Why or why not (be concise). The endonuclease DpnI, which cleaves double stranded DNA, is highly selective for methylated GATC sequences.
d) Based on you knowledge of the structure of DNA where must DpnI bind and why?